10548 Spaca3 Status: ES cell colonies screened / QC no positives
| Gene | Spaca3 |
| Chromosome | 11 |
| Deletion Start | 80,863,024 |
| Deletion End | 80,867,708 |
| Deletion Size | 4,685 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,863,922 | 80,863,943 | GGGTCTGCCTTGCTTACTACAC |
| TUP | 80,863,947 | 80,863,973 | TGGCTTCAACACAAATGCTGTGGATCA |
| TUR | 80,863,992 | 80,864,014 | TGATCTGGAAGATGCCATTGTTG |
| TDF | 80,866,834 | 80,866,855 | TGAGCGATACAAAGGAGTGGTC |
| TDP | 80,866,870 | 80,866,899 | CAGACAACAATGGAAGGACCTACATTGGCA |
| TDR | 80,866,910 | 80,866,931 | CCAAGGCTACAATGCACTCAAC |
PCR Primers
| Primer | Sequence |
| SU | GGAAGGGAGACCCCATGAG |
| SD | GAAAGCAGGAACAGACGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 346 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
