10556 Kcns1 Status: Cancelled
| Gene | Kcns1 |
| Chromosome | 2 |
| Deletion Start | 164,168,834 |
| Deletion End | 164,164,530 |
| Deletion Size | 4,305 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,168,006 | 164,168,025 | CCACCCGCTCAACCTCATTG |
| TUP | 164,167,983 | 164,168,004 | CATCGTGTCGGTGCTGCCCTTC |
| TUR | 164,167,885 | 164,167,904 | AGGCGGAACACTTGCACTAC |
| TDF | 164,164,752 | 164,164,775 | CCCATCACCATCATCTTCAACAAG |
| TDP | 164,164,728 | 164,164,749 | TTCCCACTTCTACCGGCGCCAG |
| TDR | 164,164,665 | 164,164,686 | GCTACTCAGCAAGTCCTCAAAC |
PCR Primers
| Primer | Sequence |
| SU | CAAATCCCTAGTAGATGCCAG |
| SD | CTCTTCCTCCTCTCGGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 414 bp | - | + | + |
| TUF | TUR | 141 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
