10557 Gstm4 Status: Cancelled
| Gene | Gstm4 |
| Chromosome | 3 |
| Deletion Start | 108,044,669 |
| Deletion End | 108,041,233 |
| Deletion Size | 3,437 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,043,789 | 108,043,810 | GTCAATGCTTCCTTCTGTTCAC |
| TUP | 108,043,765 | 108,043,787 | TGCTTCCGTTCCTGGTGGCGTTT |
| TUR | 108,043,743 | 108,043,762 | GCTAGGACACAGCCCATCTC |
| TDF | 108,041,769 | 108,041,792 | GAAGGCCAACAGCGTATGTAAATG |
| TDP | 108,041,744 | 108,041,768 | CCAGTGCACCTTGTCCATGCCTGTA |
| TDR | 108,041,686 | 108,041,707 | GAAAGCCACACTTCTGAACCTG |
PCR Primers
| Primer | Sequence |
| SU | CTAGAGCTCTGCATCTGCG |
| SD | AGGAAAACTAGGAGCCTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 417 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 275 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
