10558 Lgi3 Status: ES cell colonies screened / QC no positives
| Gene | Lgi3 |
| Chromosome | 14 |
| Deletion Start | 70,531,050 |
| Deletion End | 70,537,041 |
| Deletion Size | 5,992 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,531,815 | 70,531,837 | GGTGTTGGGTAATGGTGCTGATG |
| TUP | 70,531,838 | 70,531,864 | CTGGTAATGGAACCTGCTCTTGGCTGA |
| TUR | 70,531,886 | 70,531,904 | GCCTCCAGAAGCGACAGTG |
| TDF | 70,536,698 | 70,536,717 | GTGCCTTAGCCGCTACATTG |
| TDP | 70,536,719 | 70,536,741 | CGACTCCAAGATCCTGCGCTGGG |
| TDR | 70,536,751 | 70,536,772 | AGGGCTTGCACCTCAGAGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TCCATCCTTTGGCTGCTCTC |
| SD | CATAGAGAGCCTGGACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 438 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 313 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
