10559 Btnl10 Status: ES cell colonies screened / QC no positives
| Gene | Btnl10 |
| Chromosome | 11 |
| Deletion Start | 58,918,852 |
| Deletion End | 58,926,903 |
| Deletion Size | 8,052 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,918,880 | 58,918,900 | CACACCCTGGAGATGCTACTC |
| TUP | 58,918,901 | 58,918,926 | TGCCAAGCATCTTGGTCAGCTTCATC |
| TUR | 58,918,941 | 58,918,961 | GTCCTACCGTTCCCAGAAGTC |
| TDF | 58,925,985 | 58,926,008 | GACACAGACAAAGGTCACCTAGTG |
| TDP | 58,926,012 | 58,926,038 | TGGGTTCCCAGTCAAGAGCAGTTATGG |
| TDR | 58,926,059 | 58,926,079 | TGCCTGTGCTTGTGACATTTC |
PCR Primers
| Primer | Sequence |
| SU | TCACACAGAGGGGCAGCCGC |
| SD | TTCCAGAACAGGGAAAAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 309 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 306 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
