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10567 Hsd17b10 Status: ES cell colonies screened / QC no positives

Gene	Hsd17b10
Chromosome	X
Deletion Start	152,001,893
Deletion End	152,004,339
Deletion Size	2,447
Genome Build	38

MGI ID	MGI:1333871
NCBI Gene ID	15108
BAC ID	266A17
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	No

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	152,002,285	152,002,305	TCGCGGTAGTAACTGGAGGAG
TUP	152,002,308	152,002,328	TCGGGCCTTGGCCTAGCTACG
TUR	152,002,371	152,002,393	TCACCCTCTGAGTCAGGTACATC
TDF	152,003,696	152,003,716	GGGAGATGGGCCAGAATGAAC
TDP	152,003,720	152,003,745	ACCAGGGAGGCCAACGTGGAGTTATC
TDR	152,003,759	152,003,776	CCTCAAAGGCAGCCACAC

PCR Primers

Primer	Sequence
SU	TCAAGCTGGAGCCGCTAGG
SD	TCATGTATTCAACCTCAGAGC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	361 bp	-	+	+
TUF	TUR	109 bp	+	+	-
TDF	TDR	81 bp	+	+	-
NeoFwd	SD	257 bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+