10570 B4galt7 Status: Cancelled
| Gene | B4galt7 |
| Chromosome | 13 |
| Deletion Start | 55,600,146 |
| Deletion End | 55,609,382 |
| Deletion Size | 9,237 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,600,813 | 55,600,836 | GCCTTCATGCCCTCTCATACTTTG |
| TUP | 55,600,837 | 55,600,859 | CGGTTGGCCGGGAAGATCACACC |
| TUR | 55,600,874 | 55,600,897 | GCTAACCAACCAGGAAGAGAACAC |
| TDF | 55,608,917 | 55,608,937 | GGTCACTCAGCAGGTCTTCTC |
| TDP | 55,608,939 | 55,608,963 | ATAGCACCTTGCCCTGTTTGGTGGC |
| TDR | 55,608,972 | 55,608,990 | ACCCACAGTGCCCAGTTCC |
PCR Primers
| Primer | Sequence |
| SU | TCGGTTCTCCAGACAGCAG |
| SD | GTCAAGAGCCATACCTTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 313 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 296 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
