10572 Art4 Status: ES cell colonies screened / QC no positives
| Gene | Art4 |
| Chromosome | 6 |
| Deletion Start | 136,857,240 |
| Deletion End | 136,849,437 |
| Deletion Size | 7,804 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,849,789 | 136,849,812 | GCTGGTCTTGAACTCACAGAGAAC |
| TUP | 136,849,764 | 136,849,788 | CCCTTGCTTCTGGCACCTAAGTGGG |
| TUR | 136,849,743 | 136,849,761 | CCGTGACAGGGCTTCAGTC |
| TDF | 136,856,450 | 136,856,473 | GTGTAGGTGATGCTGATACAGTTC |
| TDP | 136,856,424 | 136,856,448 | CACTAACGAGCTGACCGAAGCCAGG |
| TDR | 136,856,400 | 136,856,419 | CTAGAGGGTGCCGAAAGGAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCAAAGGCGCTAGACTTC |
| SD | TGCTGCTGGGACCAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 370 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 279 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
