10573 Sat1 Status: Cancelled
| Gene | Sat1 |
| Chromosome | X |
| Deletion Start | 155,216,165 |
| Deletion End | 155,213,461 |
| Deletion Size | 2,705 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,215,736 | 155,215,756 | GGGCGGGTAATATCTGTTTCC |
| TUP | 155,215,711 | 155,215,735 | TGGCACCTCCAAAGGTCAACTCTCA |
| TUR | 155,215,658 | 155,215,679 | GTTCTCCAAAGCCATCCTCTTG |
| TDF | 155,213,842 | 155,213,859 | TGGCCTGGTCCTCCTATG |
| TDP | 155,213,821 | 155,213,841 | AGCCCTGGCTGGCCTCAAGCT |
| TDR | 155,213,750 | 155,213,770 | ACAAATGCAGAAGCCCAAATC |
PCR Primers
| Primer | Sequence |
| SU | TCCTTACTGGTCGCGATCTC |
| SD | CATACCACCTTGTTCTTCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 379 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 296 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
