10574 Hps6 Status: Cancelled
| Gene | Hps6 |
| Chromosome | 19 |
| Deletion Start | 46,003,629 |
| Deletion End | 46,006,082 |
| Deletion Size | 2,454 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,004,221 | 46,004,242 | ACCTCTTCCTGGTGCCTACTAC |
| TUP | 46,004,243 | 46,004,266 | CAACACTTGGTCTGGTGTGGCCCA |
| TUR | 46,004,276 | 46,004,295 | CCTTGCTTGGGCTCCAGATG |
| TDF | 46,005,963 | 46,005,984 | GCATCAGACCATCCAGGACAAG |
| TDP | 46,005,985 | 46,006,009 | AAGCCTGGGTACCACCTGGACAAGG |
| TDR | 46,006,040 | 46,006,061 | CCTTCGCATCTCTGTGCTCTAC |
PCR Primers
| Primer | Sequence |
| SU | TGAGCGGAGGGTTGTTAACC |
| SD | ACAGGAAACACACTCAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 595 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 318 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
