| Gene | Cyp4v3 |
| Chromosome | 8 |
| Deletion Start | 45,332,975 |
| Deletion End | 45,306,936 |
| Deletion Size | 26,040 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,332,340 | 45,332,362 | TGGCCTTTGTAGATCAATCCTTC |
| TUP | 45,332,311 | 45,332,337 | AGGAAGTCTTTCAAGGCCACTTCAGCA |
| TUR | 45,332,291 | 45,332,310 | AGGCCCTTGGCAGTAGAGTC |
| TDF | 45,307,722 | 45,307,742 | AGGCAGGTGGGAATCTCTGTG |
| TDP | 45,307,696 | 45,307,719 | TTCAAGGCCAGCCAAAGCTACACA |
| TDR | 45,307,642 | 45,307,664 | TGACACCTCTCACTCTTTCCATC |
PCR Primers
| Primer | Sequence |
| SU | CGTGGTGCAGACGCTACAG |
| SD | CTCCAGCTAGCTAACTGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | 352 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10578B-B5 | VGB6 | C57BL/6NTac | |
| 10578B-C11 | VGB6 | C57BL/6NTac | |
| 10578B-D8 | VGB6 | C57BL/6NTac | |
| 10578B-F9 | VGB6 | C57BL/6NTac | |
| 10578B-G3 | VGB6 | C57BL/6NTac | |
