10579 Bloc1s3 Status: Cancelled
| Gene | Bloc1s3 |
| Chromosome | 7 |
| Deletion Start | 19,507,598 |
| Deletion End | 19,506,970 |
| Deletion Size | 629 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,507,523 | 19,507,541 | GAGACCGACTCGGAGCTGT |
| TUP | 19,507,501 | 19,507,522 | CTGCATCCTCGTCCGAGGAGGA |
| TUR | 19,507,480 | 19,507,500 | CCCGAGGGACCCAGGTATAGT |
| TDF | 19,507,061 | 19,507,080 | CTTGCTGCCGGACATACGAG |
| TDP | 19,507,035 | 19,507,056 | CCCTGGTATGGAGCCCGAGCAG |
| TDR | 19,506,997 | 19,507,016 | GGACACAGAGTGACAGCCTA |
PCR Primers
| Primer | Sequence |
| SU | CCTAGTTCTAAGGCTTTGCC |
| SD | ATGGATAGGGGCTAAGCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 310 bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 287 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
