10580 Chrnd Status: Cancelled
| Gene | Chrnd |
| Chromosome | 1 |
| Deletion Start | 87,190,676 |
| Deletion End | 87,198,768 |
| Deletion Size | 8,093 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,191,065 | 87,191,085 | TGGCTCGAAAGGAGGACAAAG |
| TUP | 87,191,086 | 87,191,109 | TGGATGTCGCCCTAAGTCTCACCC |
| TUR | 87,191,134 | 87,191,154 | GGCTATCCCACGAAGGTCTAC |
| TDF | 87,198,346 | 87,198,367 | TCCATTGTATCCTCCTGCTCAC |
| TDP | 87,198,369 | 87,198,392 | TGTGGCTCAAAGCAGCCCTGAGTG |
| TDR | 87,198,406 | 87,198,428 | GGACAGAATGCCATTACCCTTAG |
PCR Primers
| Primer | Sequence |
| SU | CAATCTGTGACAATCCCTGC |
| SD | GATCTCTCACAGGACTGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 338 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 369 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
