10581 Polr3d Status: ES cell colonies screened / QC no positives
| Gene | Polr3d |
| Chromosome | 14 |
| Deletion Start | 70,443,112 |
| Deletion End | 70,439,438 |
| Deletion Size | 3,675 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,442,951 | 70,442,970 | CCCTTGGCGGAGTCAAGAAG |
| TUP | 70,442,922 | 70,442,948 | ACCTGCGAGCGCGTTTGTATAGAAACC |
| TUR | 70,442,874 | 70,442,896 | GGCAGCACATGAATGAACATCAC |
| TDF | 70,439,827 | 70,439,846 | TGTACCTTGGCTGACCTGAC |
| TDP | 70,439,801 | 70,439,826 | AGAGGGTCAAGTGGGCAAACTGCTCA |
| TDR | 70,439,775 | 70,439,793 | GAGTTGCACCTTCCCTGAC |
PCR Primers
| Primer | Sequence |
| SU | AGGTGAGGTTGTGATACTCG |
| SD | AGGATGCGCCTTCCATACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 281 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
