10583 Zfp365 Status: Cancelled
| Gene | Zfp365 |
| Chromosome | 10 |
| Deletion Start | 67,909,943 |
| Deletion End | 67,888,787 |
| Deletion Size | 21,176 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,909,423 | 67,909,441 | TGGTAGAGGCCGTGGACAG |
| TUP | 67,909,391 | 67,909,420 | CCATCGAGAAGAGAATCGACAAACTCACCA |
| TUR | 67,909,347 | 67,909,364 | TGCCCGGACTTCCAACAG |
| TDF | 67,889,065 | 67,889,085 | AGGGCACCAGCATTCTGTTTG |
| TDP | 67,889,035 | 67,889,060 | CATCCTGAGATGAGGGCCCATTTCCA |
| TDR | 67,888,992 | 67,889,010 | TCGCTCATCCTTGGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | CCAACTAGAAAGGCAGACAG |
| SD | AGAACTTCACAGTCACATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 380 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
