10585 Arl11 Status: ES cell colonies screened / QC no positives
| Gene | Arl11 |
| Chromosome | 14 |
| Deletion Start | 61,310,726 |
| Deletion End | 61,311,273 |
| Deletion Size | 548 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,310,759 | 61,310,778 | CCAGAGGTCACAAGGCAGAG |
| TUP | 61,310,781 | 61,310,806 | CCAGGTGGTAATGATGGGCCTCGACT |
| TUR | 61,310,857 | 61,310,876 | CCAACAGTGGGTAGGGTGTC |
| TDF | 61,311,083 | 61,311,102 | GCGTCCCCTTCTTGGTACTG |
| TDP | | | |
| TDR | 61,311,171 | 61,311,191 | TCCCAGCAATGCTTTTGGAAC |
PCR Primers
| Primer | Sequence |
| SU | TCCCTAAAATGACTTGTTTTG |
| SD | TTCCCTCTGACTATTGTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 245 bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 332 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
