10586   Cyp11b1     Status: ES cell colonies screened / QC positives

GeneCyp11b1
Chromosome15
Deletion Start74,841,609
Deletion End74,835,718
Deletion Size5,892
Genome Build38
MGI IDMGI:88583
NCBI Gene ID110115
BAC ID310K23
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,841,00074,841,018GCTCAGGTGGAGGGCATTC
TUP74,840,97474,840,999CACTAACAGCAGGGCTCTGTGCTTCA
TUR74,840,92474,840,944GGCAACGTCACAAACACAATC
TDF74,835,93474,835,956AGTCACAATACTGGGCAGTTCAG
TDP74,835,90674,835,930AAGATGTGCCAGTGCTCTGCTCTGC
TDR74,835,88674,835,904GGCTGCATCTGGCCTACTC

PCR Primers

PrimerSequence
SUAGAAAGAAGGCTCAAACGAC
SDAACAAAGATATTTTAGAAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev384 bp-++
TUFTUR95 bp++-
TDFTDR71 bp++-
NeoFwdSD362 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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