10588 Icam4 Status: Cancelled
| Gene | Icam4 |
| Chromosome | 9 |
| Deletion Start | 21,029,437 |
| Deletion End | 21,030,494 |
| Deletion Size | 1,058 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,029,536 | 21,029,554 | AAAGTCCTCCCGCGCCTTC |
| TUP | 21,029,555 | 21,029,578 | CGTGACCTCAGCACCTTTCTGGGT |
| TUR | 21,029,581 | 21,029,603 | GGCCTCTAGCTCTGGATTAAGAC |
| TDF | 21,030,034 | 21,030,053 | GGTGGTGAGCCTGAGAAGAG |
| TDP | 21,030,055 | 21,030,083 | TGGCCGAGTGATTTATCATGAAAGCCTGG |
| TDR | 21,030,117 | 21,030,137 | CCCGCATCACGTAGGTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGACTTCTTGGGGCTTACG |
| SD | CCCCATGCTGGCCTTGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 330 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | 335 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
