10590 Bcs1l Status: Germline transmission achieved
| Gene | Bcs1l |
| Chromosome | 1 |
| Deletion Start | 74,636,136 |
| Deletion End | 74,638,647 |
| Deletion Size | 2,512 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CCTGGTTGCTTAGCTGGCTTAC |
| TUP | | | CCGACACAGCACCCGCACTCAG |
| TUR | | | GTGGAGATCCGGCCACTTTC |
| TDF | | | GACTTCTCACATTCCTGTTATCCTC |
| TDP | | | CCCTATAAAGTACCAAGGACTAGGCCGCCT
|
| TDR | | | CCAAAGCATTAAGCAGTCCAC |
PCR Primers
| Primer | Sequence |
| SU | GAGCAAAATGGATTGAAGAG |
| SD | TGTGCCACAACACAGGGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| NeoFwd | SD | 356 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10590A-B8 | VGB6 | C57BL/6NTac | |
| 10590A-D3 | VGB6 | C57BL/6NTac | |
| 10590A-H1 | VGB6 | C57BL/6NTac | |
| 10590B-E7 | VGB6 | C57BL/6NTac | |
| 10590B-A12 | VGB6 | C57BL/6NTac | |
| 10590B-A7 | VGB6 | C57BL/6NTac | |
