10594 Pla2g2a Status: Cancelled
| Gene | Pla2g2a |
| Chromosome | 4 |
| Deletion Start | 138,832,675 |
| Deletion End | 138,834,892 |
| Deletion Size | 2,218 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,833,137 | 138,833,156 | CAGCCGAGGTCAGCACATTG |
| TUP | 138,833,159 | 138,833,186 | AGCTCTGCTCTGGACCACTCCTAAGTTC |
| TUR | 138,833,215 | 138,833,235 | GGCATCCCAGCATTTCCTTTG |
| TDF | 138,834,294 | 138,834,312 | CAGCCGGGCTCACTTACAC |
| TDP | 138,834,316 | 138,834,341 | CAGACCTGTGAGCAAGAAGGCAGGAC |
| TDR | 138,834,378 | 138,834,398 | GAACCCAGGCCCTCACCTATG |
PCR Primers
| Primer | Sequence |
| SU | CAATTCTGGGGAATGAGTCC |
| SD | AGGAAGTTGGATGCCAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 428 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
