10597 Col9a3 Status: Cancelled
| Gene | Col9a3 |
| Chromosome | 2 |
| Deletion Start | 180,598,263 |
| Deletion End | 180,621,420 |
| Deletion Size | 23,158 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,598,931 | 180,598,953 | TGATGTGAGTTGGGAGAGGAATG |
| TUP | 180,598,958 | 180,598,980 | AGGTGCCCTGAGACTCCTGGCCT |
| TUR | 180,598,987 | 180,599,005 | GAAGAGGCGCTTGGGTTTG |
| TDF | 180,621,176 | 180,621,197 | TGGCTGCTATTATGGGCTTCTC |
| TDP | 180,621,204 | 180,621,232 | CCTAACTGCACTCTCCTGGTTTCTAGGGC |
| TDR | 180,621,255 | 180,621,277 | GAGCACCATCTCGACCATCTTTG |
PCR Primers
| Primer | Sequence |
| SU | AGGAGTGGGAGAGAAAAGAG |
| SD | CCCAGATGCTGGGTTTAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 419 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 357 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
