10600   Mc2r     Status: ES cell colonies screened / QC no positives

GeneMc2r
Chromosome18
Deletion Start68,408,217
Deletion End68,407,308
Deletion Size910
Genome Build38
MGI IDMGI:96928
NCBI Gene ID17200
BAC ID311M20
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 18

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF68,407,86768,407,890ATCCTCTCTTTGCTGGGCTCTATC
TUP68,407,84168,407,865TCAGCCTGTCTGTCATTGCAGCTGA
TUR68,407,81368,407,834CAGGGCATGGAAGATGGTGATG
TDF68,407,60368,407,621CCGCTCCCATGCTAGGAAG
TDP68,407,56668,407,592TTCCTAGAACCAACATGAAGGGTGCCA
TDR68,407,53668,407,560TGAAGACTCCAAGAAGGATGGTTAG

PCR Primers

PrimerSequence
SUTCATTCTGCAATATGTGTTC
SDACAGGTATCCACAGAAGAAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev255 bp-++
TUFTUR78 bp++-
TDFTDR86 bp++-
NeoFwdSD355 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image