10605   Gnb3     Status: ES cell colonies screened / QC positives

GeneGnb3
Chromosome6
Deletion Start124,839,717
Deletion End124,834,636
Deletion Size5,082
Genome Build38
MGI IDMGI:95785
NCBI Gene ID14695
BAC ID365B7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF124,839,393124,839,411CCGCAGGATGCCAGGAAAG
TUP124,839,367124,839,390TGTGCGGACATCACTCTGGCTGAG
TUR124,839,344124,839,362CTCAGATGGGCAGAAGGTC
TDF124,835,489124,835,510CCTCACCACTGATCCCTAACTG
TDP124,835,466124,835,487TGCCTGACAGGAGCCTTGGGAA
TDR124,835,428124,835,447GGGAACGCGGTCTTAGCTTC

PCR Primers

PrimerSequence
SUCGGAAACCTGGGAGAAGTC
SDGTGGAGAGGAGAGGCCATG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev349 bp-++
TUFTUR68 bp++-
TDFTDR83 bp++-
NeoFwdSD272 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10605A-A7VGB6C57BL/6NTac
10605A-G11VGB6C57BL/6NTac
10605A-E6VGB6C57BL/6NTac
10605A-A8VGB6C57BL/6NTac
10605A-B2VGB6C57BL/6NTac
10605A-B1VGB6C57BL/6NTac