10613 Gpr27 Status: Cancelled
| Gene | Gpr27 |
| Chromosome | 6 |
| Deletion Start | 99,692,665 |
| Deletion End | 99,693,835 |
| Deletion Size | 1,171 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,692,843 | 99,692,861 | GCCGTACTACCTGCTGCTC |
| TUP | 99,692,863 | 99,692,882 | ACCTGTGCCTGGCCGACGGG |
| TUR | 99,692,972 | 99,692,990 | CAGGAAGGCCAGCAGCTTG |
| TDF | 99,693,502 | 99,693,521 | TGGTGCTGGAGGAGTTCAAG |
| TDP | 99,693,522 | 99,693,550 | ACGGAGAAGAGGCTGTGCAAGATGTTCTA |
| TDR | 99,693,554 | 99,693,573 | GCAGGAAGAGCAGCGTGATG |
PCR Primers
| Primer | Sequence |
| SU | AGGGAGTGGCCGTCGCCT |
| SD | TCACACGTGGAAGAAAAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 148 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
