10614 Ffar1 Status: ES cell clone microinjected
| Gene | Ffar1 |
| Chromosome | 7 |
| Deletion Start | 31,646,486 |
| Deletion End | 31,645,545 |
| Deletion Size | 942 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,646,354 | 31,646,373 | TCCCAGCTTGGTCTACACTC |
| TUP | 31,646,330 | 31,646,353 | TCCATCTGGGCTGCTCTGATCTCC |
| TUR | 31,646,290 | 31,646,309 | AGGGCCTCCACAGCCTTCAG |
| TDF | 31,645,917 | 31,645,940 | CTCAGTTTCTCCATTCTGCTCTTC |
| TDP | 31,645,893 | 31,645,915 | TTCTGCCCTTGGTCATCACTGCC
|
| TDR | 31,645,832 | 31,645,850 | GCTTCCGTTTGTGGCTCAG |
PCR Primers
| Primer | Sequence |
| SU | CCCGAGTTCGGTTGCTCTC |
| SD | CAGAAGCAGTTGGGAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 299 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
