10615 Rab37 Status: Cancelled
| Gene | Rab37 |
| Chromosome | 11 |
| Deletion Start | 115,154,184 |
| Deletion End | 115,160,779 |
| Deletion Size | 6,596 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,154,752 | 115,154,772 | GACTCACAGCTCCTTCCCAAC |
| TUP | 115,154,776 | 115,154,796 | CCAGGCTGCATGTAGCCAGCC |
| TUR | 115,154,819 | 115,154,841 | GCCATGTTCTTTCTCAGCTTCTG |
| TDF | 115,159,819 | 115,159,841 | CAGCTCCATAACCACCTAAATCC |
| TDP | 115,159,842 | 115,159,864 | TTCCGGCAGAGGACTCTGAGGTC |
| TDR | 115,159,886 | 115,159,906 | GACAGGCAGAAGCCACAAGAC |
PCR Primers
| Primer | Sequence |
| SU | AACTAGGCTAATGTCTCTCAG |
| SD | ACACTCTTGCTAGGAGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
