10618 Gpr141 Status: Cancelled
| Gene | Gpr141 |
| Chromosome | 13 |
| Deletion Start | 19,752,600 |
| Deletion End | 19,751,641 |
| Deletion Size | 960 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,752,449 | 19,752,469 | GAACTCACGTTCAGTGACCAC |
| TUP | 19,752,423 | 19,752,446 | TGGCTGTCATCAACCTCGTGGTGG |
| TUR | 19,752,395 | 19,752,414 | GGCACCGTCAGTAGGAAGAC |
| TDF | 19,752,007 | 19,752,028 | TGCGTTGATTCTCTTGGGTTTC |
| TDP | 19,751,978 | 19,752,006 | CAGGTCTTCATCACATTGTCCATGGTGCG |
| TDR | 19,751,939 | 19,751,962 | CAGAACTCCTGGTGGGATAGTAAG |
PCR Primers
| Primer | Sequence |
| SU | TCAGTATCACACTGACTGATG |
| SD | CAGCACCCAGTAGACACATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 338 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 339 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
