| Gene | Rhox11 |
| Chromosome | X |
| Deletion Start | 38,084,974 |
| Deletion End | 38,076,393 |
| Deletion Size | 8,582 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,084,795 | 38,084,813 | AGGCGAGCATAGCTGTGTC |
| TUP | 38,084,768 | 38,084,791 | CCTAACACTCAGGACACTGGGCGA |
| TUR | 38,084,732 | 38,084,750 | GGTCTCGGCCACCTTACTG |
| TDF | 38,076,650 | 38,076,672 | AACACCAGAACATTGACACCCAA |
| TDP | 38,076,618 | 38,076,647 | CAGCCCTCTCTCTCTTCTCTTCAGTTAGCA |
| TDR | 38,090,104 | 38,090,124 | TCTGTGGAAAGGACACCCAAG |
PCR Primers
| Primer | Sequence |
| SU | CAACTAGGGCTTCACTTCTC |
| SD | AGAGCTGCTGTTTTGTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 424 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 13475 bp | + | + | - |
| NeoFwd | SD | 14188 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10620A-F4 | VGB6 | C57BL/6NTac | |
| 10620C-D2 | VGB6 | C57BL/6NTac | |
| 10620C-B2 | VGB6 | C57BL/6NTac | |
