10625 Fam171a2 Status: Cancelled
| Gene | Fam171a2 |
| Chromosome | 11 |
| Deletion Start | 102,447,290 |
| Deletion End | 102,437,132 |
| Deletion Size | 10,178 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,446,712 | 102,446,730 | ATGGCAGGGCTGTGGTCAC |
| TUP | 102,446,688 | 102,446,709 | AGCCATGCTGAGCTGGCTGGCT |
| TUR | 102,446,626 | 102,446,646 | GCAAGAGACAGGCTGGACAAG |
| TDF | 102,438,075 | 102,438,094 | TCACCATTCCGGTGCTGTTC |
| TDP | 102,438,053 | 102,438,074 | AACGAGTCCACCATGGCGCAGC |
| TDR | 102,438,005 | 102,438,024 | CCAGTTCCAGCAGCTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | ACCACATCTCAAGAGCTCTC |
| SD | TAGCAGCTGAGGCTGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 685 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 112 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
