10626 Tnk1 Status: Cancelled
| Gene | Tnk1 |
| Chromosome | 11 |
| Deletion Start | 69,857,071 |
| Deletion End | 69,851,602 |
| Deletion Size | 5,470 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,856,769 | 69,856,792 | CCTCACCCTCTAATTCTGGTCTTC |
| TUP | 69,856,744 | 69,856,767 | CCAGCCCAGCGCAGACTTAATGAA |
| TUR | 69,856,666 | 69,856,685 | GCCCTCAACGACCACCATAG |
| TDF | 69,852,055 | 69,852,076 | TCCTCACATAATCACCGCACTG |
| TDP | 69,852,030 | 69,852,051 | CACCTGGAGCCAGCAAAGCCAC |
| TDR | 69,851,979 | 69,851,998 | ACAACCTTCCTCTGCCACTC |
PCR Primers
| Primer | Sequence |
| SU | GATATCTTGCATCCCTGGGC |
| SD | TTCAAGTCCTGTTCAATTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 266 bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 356 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
