10629 Gp49a Status: Cancelled
| Gene | Gp49a |
| Chromosome | 10 |
| Deletion Start | 51,491,000 |
| Deletion End | 51,485,767 |
| Deletion Size | 5,234 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,481,640 | 51,481,662 | GTGCAGCTCATCCACACTATTTG |
| TUP | 51,481,670 | 51,481,698 | CATTCTGATACAGGAAGGAAAGCATGGCC |
| TUR | 51,481,703 | 51,481,720 | TGTGAGTCCAGGGTCCAC |
| TDF | 51,485,197 | 51,485,215 | TGTGGCTGCATCCCTACAG |
| TDP | 51,495,501 | 51,495,527 | CAGCTGAGATCATCTCTTCCCTCTGGC |
| TDR | 51,495,560 | 51,495,580 | CTTGGAGTGGGTGAGGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TAACCCCAGGATATGTGTCC |
| SD | CTGCTTGCTGGTTTCAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 360 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 10384 bp | + | + | - |
| NeoFwd | SD | 10572 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
