10633 Serpinb9g Status: Cancelled
| Gene | Serpinb9g |
| Chromosome | 13 |
| Deletion Start | 33,486,515 |
| Deletion End | 33,495,300 |
| Deletion Size | 8,786 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,486,648 | 33,486,668 | GCCATGGTTCTCTTGGGTGCT |
| TUP | 33,486,669 | 33,486,697 | AAGGGAGACACTGCAGTCCAGATATGTCA |
| TUR | 33,486,702 | 33,486,726 | CAAGGGTATGGTTTTACAGGGAACG |
| TDF | 33,494,366 | 33,494,394 | CCATCCTCTGAATTGTTGGTAGTAAAATG |
| TDP | 33,494,409 | 33,494,436 | TGTCTTTCAAAGCAAAAGAGCTCCCTGC |
| TDR | 33,494,453 | 33,494,480 | CTCTATTGAAGTGAAGCCACAATTAGAA |
PCR Primers
| Primer | Sequence |
| SU | TTCCGTCTATCTATGCAGATG |
| SD | TGAGGACTACCTTTGCCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 353 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
