| Gene | Col6a6 |
| Chromosome | 9 |
| Deletion Start | 105,788,952 |
| Deletion End | 105,774,151 |
| Deletion Size | 14,802 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,788,017 | 105,788,037 | CGTGTACGCAGGAGGAGAAAG |
| TUP | 105,787,982 | 105,788,009 | TGGCCATATAACAACCCTCCCTGAGCAG |
| TUR | 105,787,954 | 105,787,972 | AAGGGAAGGCTGGCTGGTC |
| TDF | 105,774,429 | 105,774,449 | CCGAGGAGCTGAGACACAAAG |
| TDP | 105,774,402 | 105,774,425 | TGGACATCTACTCGGTGGGCATCG |
| TDR | 105,774,377 | 105,774,399 | ACCAATTCCTGGTCATCCACATC |
PCR Primers
| Primer | Sequence |
| SU | AACATCTCGTGTTTTCTCAGC |
| SD | AACCTATTGCCAGGCAACCA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 390 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10634C-A4 | VGB6 | C57BL/6NTac | |
| 10634C-B1 | VGB6 | C57BL/6NTac | |
| 10634C-F7 | VGB6 | C57BL/6NTac | |
| 10634C-A10 | VGB6 | C57BL/6NTac | |
