| Gene | Amigo3 |
| Chromosome | 9 |
| Deletion Start | 108,053,365 |
| Deletion End | 108,054,906 |
| Deletion Size | 1,542 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,053,525 | 108,053,544 | GCCGTGGGTTACAGGACTTG |
| TUP | 108,053,552 | 108,053,577 | CACTGCCTACCACTGCTGCAGAACTC |
| TUR | 108,053,587 | 108,053,605 | GGCGTTTGAGTGCGTTGTG |
| TDF | 108,054,505 | 108,054,525 | CCCGAGCCAGAGACTTTCAAC |
| TDP | 108,054,528 | 108,054,551 | AGGCTTTACCACCCTGCTGGGCTG |
| TDR | 108,054,568 | 108,054,590 | GCAAACAAGTAGAGCAACACCAG |
PCR Primers
| Primer | Sequence |
| SU | CTAGTACTTAGAATCCAGTCAG |
| SD | TGGGACATTTGGTCACTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 283 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10635A-F10 | VGB6 | C57BL/6NTac | |
| 10635C-D7 | VGB6 | C57BL/6NTac | |
| 10635D-C8 | VGB6 | C57BL/6NTac | |
