| Gene | Ppp3r2 |
| Chromosome | 4 |
| Deletion Start | 49,681,945 |
| Deletion End | 49,681,106 |
| Deletion Size | 840 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,681,823 | 49,681,843 | GGCTCCCTGAGCATAGAAGAG |
| TUP | 49,681,799 | 49,681,822 | TTCATGAGGCTGCCTGAGCTGCAG |
| TUR | 49,681,757 | 49,681,777 | TGTGTCGAAGATGTCGATCAC |
| TDF | 49,681,315 | 49,681,335 | CGACTAACCCGGAAGTGTTTC |
| TDP | 49,681,288 | 49,681,314 | TCTCTACCGCTATCACCCAGGAAGTGT |
| TDR | 49,681,247 | 49,681,266 | TCCCCTCTTTGCCTGTGTAG |
PCR Primers
| Primer | Sequence |
| SU | GAGGACAGCCCAGTTACAG |
| SD | GTTTTCACATGAACACTGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 338 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 372 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10637A-A6 | VGB6 | C57BL/6NTac | |
| 10637A-B3 | VGB6 | C57BL/6NTac | |
| 10637C-B5 | VGB6 | C57BL/6NTac | |
| 10637C-B6 | VGB6 | C57BL/6NTac | |
| 10637C-C11 | VGB6 | C57BL/6NTac | |
| 10637C-E8 | VGB6 | C57BL/6NTac | |
| 10637C-B10 | VGB6 | C57BL/6NTac | |
