10639   Sp6     Status: Cancelled

GeneSp6
Chromosome11
Deletion Start97,021,466
Deletion End97,022,593
Deletion Size1,128
Genome Build38
MGI IDMGI:1932575
NCBI Gene ID83395
BAC ID
BAC Library
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,021,69497,021,712TGCGAGGACCTGGAAAGTG
TUP97,021,71397,021,734ACAGTCCCTTGGCACCGGGACC
TUR97,021,80697,021,824CACCACGAGCCATCCTCTG
TDF97,022,24597,022,265CGGCAAGGCATACGCTAAGAC
TDP97,022,26897,022,288CGCATCTGAAGGCGCACCTGC
TDR97,022,30197,022,319CACACGAAGGGTCGGTCAC

PCR Primers

PrimerSequence
SUTGACCTGGACTTTCACATCC
SDGCGCTCCATCCCAACTCTTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev362 bp-++
TUFTUR131 bp++-
TDFTDR75 bp++-
NeoFwdSD356 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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