10642 Lzts1 Status: ES cell colonies screened / QC no positives
| Gene | Lzts1 |
| Chromosome | 8 |
| Deletion Start | 69,140,950 |
| Deletion End | 69,135,469 |
| Deletion Size | 5,482 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,140,042 | 69,140,061 | CAGTTCACCCGCTCAGTCTC |
| TUP | 69,140,022 | 69,140,041 | CGTGCTCACGCTGTGGCAGA |
| TUR | 69,139,964 | 69,139,984 | TTCCGTTCCCAAGCATTTCAG |
| TDF | 69,135,750 | 69,135,769 | GCCGAGCTGAAGGAGGAGAG |
| TDP | 69,135,724 | 69,135,748 | CAAGGCCATGACCAGATGTCCTCGG |
| TDR | 69,135,702 | 69,135,723 | ACCAACCGCTCATGCTGGAATC |
PCR Primers
| Primer | Sequence |
| SU | AGCCTCTGACGTACCCCTCG |
| SD | GACCAAAGTTGAGAGAAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 365 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
