10643 Dhrs3 Status: ES cell colonies screened / QC no positives
| Gene | Dhrs3 |
| Chromosome | 4 |
| Deletion Start | 144,893,518 |
| Deletion End | 144,924,020 |
| Deletion Size | 30,522 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 144,893,945 | 144,893,962 | TTGGCAGGCCCTGAGTTG |
| TUP | 144,893,971 | 144,893,995 | CCCTGGGATTGGTATCTCGCTGGCT |
| TUR | 144,894,007 | 144,894,031 | TGGGTATTCTAGGTATGGTAGTTGG |
| TDF | 144,923,630 | 144,923,650 | GTGTCCAGTGTGCCAGGAATG |
| TDP | 144,923,651 | 144,923,676 | CTAGAGGGCATTGGAGAATGAGCCCA |
| TDR | 144,923,711 | 144,923,730 | GCCCACTAGCAGGGAGTATC |
PCR Primers
| Primer | Sequence |
| SU | TCCCATTTTTCCTCGCCCTG |
| SD | ACAGATGAACTAAGCAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 334 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | 332 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
