10645   Fnd3c2     Status: Cancelled

GeneFnd3c2
ChromosomeX
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:2685621
NCBI Gene ID331491
BAC ID
BAC Library
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF106,252,149106,252,174AACTAATGATGGCCTCAGACCTTCTG
TUP106,252,117106,252,144TGGCAGCACTTCTGAAAACAATCATGGA
TUR106,252,096106,252,116TCCCATGTTAGCCTCTGTTTC
TDF106,235,957106,235,981GAACACTTCCTTTTCCTTTTCCAAC
TDPTCATGCAAATTCACGCT
TDR106,235,906106,235,926ATATCGGCGTCCTGCACAGAC

PCR Primers

PrimerSequence
SUTGGAAGAGTGAGCCATAACC
SDACATTTTTATTACTTTATAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR79 bp++-
TDFTDR76 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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