10646 Ifnz Status: Cancelled
| Gene | Ifnz |
| Chromosome | 4 |
| Deletion Start | 88,806,169 |
| Deletion End | 88,806,736 |
| Deletion Size | 568 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,806,381 | 88,806,403 | GGAACTAACATCACACCGATGAC |
| TUP | 88,806,406 | 88,806,431 | TGGGAGAAACCACCAGTTGCTACTCC |
| TUR | 88,806,438 | 88,806,456 | GGTGGAGGACCTGCTTGAG |
| TDF | 88,783,197 | 88,783,217 | ATCCGCACCTACTTCCGAGGT |
| TDP | 88,806,619 | 88,806,644 | TCTTCCGCTATCTGAAGGCAAAGGCA |
| TDR | 88,783,245 | 88,783,262 | CCAGGAGCAAGCGCTGTA |
PCR Primers
| Primer | Sequence |
| SU | GCCCGGGAGAGAGGCAGGAG |
| SD | TCAGAAGCCACCTCAAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 403 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
