10650 Creb3 Status: ES cell clone microinjected
| Gene | Creb3 |
| Chromosome | 4 |
| Deletion Start | 43,575,667 |
| Deletion End | 43,579,886 |
| Deletion Size | 4,220 |
| Genome Build | 37 |
|
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,575,768 | 43,575,786 | TTTGTGGGCTGCGACTGAG |
| TUP | 43,575,787 | 43,575,806 | CCGGACGTGAAGGCTCCGCT |
| TUR | 43,575,810 | 43,575,830 | TCGGAAGGTGACAGCTCTAAG |
| TDF | 43,578,893 | 43,578,915 | CACAGCATGAGTGGAGAGGGTAG |
| TDP | 43,578,916 | 43,578,941 | CTAAGACAGGAGGCCTTCATCGTGCC
|
| TDR | 43,578,968 | 43,578,989 | AAGAGACGAGAGGAGACGGTAG |
PCR Primers
| Primer | Sequence |
| SU | AGGAGCCACTTCCGAGTTG |
| SD | ACAAGGCACGGGACTAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 43 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 330 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10650B-C5 | VGB6 | C57BL/6NTac | |
| 10650B-B1 | VGB6 | C57BL/6NTac | |
