10651 Murc Status: ES cell colonies screened / QC no positives
| Gene | Murc |
| Chromosome | 4 |
| Deletion Start | 48,663,805 |
| Deletion End | 48,672,731 |
| Deletion Size | 8,948 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,663,869 | 48,663,892 | GCTACGTTGTCAACAAGCTGTTTG |
| TUP | 48,663,893 | 48,663,917 | AGAAGACCCGGAAAGTCAGCGCTCA |
| TUR | 48,663,943 | 48,663,962 | ACACGAACCTGTTGCTTCTC |
| TDF | 48,672,333 | 48,672,351 | CAGTGCCGCTCCCTCAAAG |
| TDP | 48,672,359 | 48,672,388 | TTAAGATTCGGAGCCTTAGGAAAGCGAAGG |
| TDR | 48,672,406 | 48,672,427 | CCATCCCTCTGTCTACCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | TTGCCATAGGACACCTTCTG |
| SD | TGTTCCTGTGTTATGAATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 351 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
