10652 Spinlw1 Status: Cancelled
| Gene | Spinlw1 |
| Chromosome | 2 |
| Deletion Start | 164,593,513 |
| Deletion End | 164,588,485 |
| Deletion Size | 5,029 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,593,368 | 164,593,392 | GAAAGGGAAGTTTACTGGAAGGAAG |
| TUP | 164,593,335 | 164,593,364 | CAAAGAACAATTCCTCAGGTGTGGGTCACC |
| TUR | 164,593,312 | 164,593,331 | CACCCAGGAAGGAGCTGTAG |
| TDF | 164,588,698 | 164,588,720 | AACTCCAGGCTCCAGAAACAAAC |
| TDP | 164,588,675 | 164,588,697 | CCAGCCCGAGATGCTCAGCTCTG |
| TDR | 164,588,621 | 164,588,641 | CCCAGCGTATCCTCTGTTCAG |
PCR Primers
| Primer | Sequence |
| SU | GAGTACACTTTGGGCACTAC |
| SD | GATGTGTGGACCACTCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 401 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
