10659   Tgfb1i1     Status: ES cell colonies screened / QC no positives

GeneTgfb1i1
Chromosome7
Deletion Start128,248,218
Deletion End128,253,367
Deletion Size5,150
Genome Build38
MGI IDMGI:102784
NCBI Gene ID21804
BAC ID54F24
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF128,248,763128,248,785GGCAAAGAAGGGCAGAGTCTAAG
TUP128,248,790128,248,817TCAAACTGTCCGAGGAGCCATTAAAGGA
TUR128,248,826128,248,844GTCCACCCTCCCAGTGAAG
TDF128,252,499128,252,517ACCCACTGGCACCCAGAAC
TDP128,252,519128,252,541TTTCTGCTGCGTCAGCTGCGGAG
TDR128,252,553128,252,573GTTTCAGCTCTCACCCTCTTC

PCR Primers

PrimerSequence
SUGTCAAGGGAGAGGTCTGATC
SDCAAGAGAACCAGAATCATGTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev430 bp-++
TUFTUR82 bp++-
TDFTDR75 bp++-
NeoFwdSD347 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image