10661 Taar9 Status: Cancelled
| Gene | Taar9 |
| Chromosome | 10 |
| Deletion Start | 24,109,531 |
| Deletion End | 24,108,362 |
| Deletion Size | 1,170 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,109,188 | 24,109,208 | GTGTTTCGACACCTCCTTCTG |
| TUP | 24,109,157 | 24,109,186 | TTTGCGTCTCTGTTTCATTTATGCTGCATC |
| TUR | 24,109,107 | 24,109,126 | TTGGTCGGATAGGTCAGTGG |
| TDF | 24,108,522 | 24,108,543 | TCAGGGCTGATTCATCAACAAC |
| TDP | 24,108,489 | 24,108,513 | TCTCTGAAGAGGCAGGTGCAGGTTA |
| TDR | 24,108,436 | 24,108,460 | TTACCTCTGACCTTAAACCCTAGTC |
PCR Primers
| Primer | Sequence |
| SU | GACAATGTACATTTACTTAGTAAG |
| SD | ACTTCTCATGCAACATATGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 782 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 399 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
