| Gene | Cbr2 |
| Chromosome | 11 |
| Deletion Start | 120,731,969 |
| Deletion End | 120,729,703 |
| Deletion Size | 2,267 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,731,081 | 120,731,099 | TGGACGGGACACTGTGAAG |
| TUP | 120,731,057 | 120,731,077 | CTGCACGCCTCAGGAGCCAAA |
| TUR | 120,731,017 | 120,731,036 | GGCTGACCAGGTCTGAGTTG |
| TDF | 120,729,797 | 120,729,816 | ACGTCGTCAACAGCATCCTC |
| TDP | 120,767,293 | 120,767,314 | CCTGCTCAGCGACAGAAGTGCC |
| TDR | 120,767,336 | 120,767,354 | GGTAGCCAGCATCCACCAG |
PCR Primers
| Primer | Sequence |
| SU | GTCTCCGGTTTCACAGCTCC |
| SD | AGTCCTGTGCAATGGCAATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 37558 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10662A-G5 | VGB6 | C57BL/6NTac | |
| 10662C-D6 | VGB6 | C57BL/6NTac | |
