10663   Ifna2     Status: ES cell colonies screened / QC no positives

GeneIfna2
Chromosome4
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:107666
NCBI Gene ID15965
BAC ID441P14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF88,683,57688,683,596CCCCTGGAGAAGGTGGATAAC
TUP88,649,07788,649,101CAGCAGATCCAGAAGGCTCAAGCCA
TUR88,683,50188,683,522TGATGTGAAGAGGTTCAAGGTC
TDF88,683,46088,683,483TTGGAATACAACCCTCCTAGACTC
TDP88,683,43488,683,456CTGCAATGACCTCCACCAGCAGC
TDR88,683,40488,683,423GCTGCATCAGACAGGTTTGC

PCR Primers

PrimerSequence
SUTAGAGTAGCTTTCTGAGGGC
SDAAAATATTTGGTAATGTTAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR96 bp++-
TDFTDR80 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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