| Gene | Rnf186 |
| Chromosome | 4 |
| Deletion Start | 138,967,316 |
| Deletion End | 138,967,993 |
| Deletion Size | 678 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,967,494 | 138,967,513 | TCTGTGCCGTATGCCTGAAG |
| TUP | 138,967,516 | 138,967,543 | TCTGCTATATGTGCAGGAAGACACCTGG |
| TUR | 138,967,566 | 138,967,585 | CGGGACAGCAGTGACCTTTC |
| TDF | 138,967,807 | 138,967,831 | TGTCCTTATCCTGCCTTTCATCTAC |
| TDP | 138,967,839 | 138,967,860 | TCATCCGGTGGGTGTTGGCCTT |
| TDR | 138,967,880 | 138,967,902 | CAGCAGAATAGGGTGGACATCAG |
PCR Primers
| Primer | Sequence |
| SU | AAGATGCCAGACATCGGTTG |
| SD | ACTGTTCCAGCACAGAATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 300 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10668A-A6 | VGB6 | C57BL/6NTac | |
| 10668A-B2 | VGB6 | C57BL/6NTac | |
| 10668A-B6 | VGB6 | C57BL/6NTac | |
| 10668A-C8 | VGB6 | C57BL/6NTac | |
| 10668A-E6 | VGB6 | C57BL/6NTac | |
| 10668A-F2 | VGB6 | C57BL/6NTac | |
