| Gene | Inpp5j |
| Chromosome | 11 |
| Deletion Start | 3,504,745 |
| Deletion End | 3,494,640 |
| Deletion Size | 10,106 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,504,044 | 3,504,066 | TTCCTCTGTTCTCCCTGGTAAAG |
| TUP | 3,504,014 | 3,504,040 | ACTTTCTCCTGGCCTCTGGAGACTGGG |
| TUR | 3,503,981 | 3,504,000 | GACACCTGCCTTGTCCATTC |
| TDF | 3,495,617 | 3,495,636 | TGGGCTTCCGTCACTGTAAG |
| TDP | 3,495,589 | 3,495,613 | TATGTGGCTTACGTCTGGGCCAAGC |
| TDR | 3,495,559 | 3,495,583 | CCTGGTAGATATTCCCATCCACTTC |
PCR Primers
| Primer | Sequence |
| SU | GGTCTCCTTCAGCATGCAGG |
| SD | AAGCTGGAGAATTTAATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 375 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 357 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10673A-C8 | VGB6 | C57BL/6NTac | |
| 10673A-A8 | VGB6 | C57BL/6NTac | |
