10676 Ifna7 Status: ES cell colonies screened / QC one positive
| Gene | Ifna7 |
| Chromosome | 4 |
| Deletion Start | 88,835,506 |
| Deletion End | 88,817,070 |
| Deletion Size | 18,437 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,816,447 | 88,816,465 | GCTCAAGCCCTCTGTGTTC |
| TUP | 88,816,468 | 88,816,492 | CATGAGCTGACCCAGCAGATCCTGA |
| TUR | 88,820,240 | 88,820,258 | CCAAGCAGCAGATGAGTCC |
| TDF | 88,816,771 | 88,816,793 | CTGGCAAGATTGAGTGAGAAGAA |
| TDP | 88,816,802 | 88,816,831 | TCCTGAGACAAAGTAGAGAGGATCTCCAGG |
| TDR | 88,816,844 | 88,816,866 | GGTAGAATCTGGACAGAGAGGTC |
PCR Primers
| Primer | Sequence |
| SU | GAATTGGAATGAGAGGGGAAG |
| SD | CAGAAGATAACTGTGCACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 19585 bp | - | + | + |
| TUF | TUR | 3812 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
